A brain-tumor neural circuit controls breast cancer progression in mice

CEA corticotropin-releasing hormone-expressing neurons nt-vpnb3tg80r synapses onto PGRNl (Paragigantocellular reticular nucleus, lateral part) MBA:978 · Mouse

Cre-dependent helper AAVs plus a Dbh-Cre virus were placed in the LPGi and then pseudotyped, glycoprotein-deleted rabies virus was injected at the same site; retrogradely labelled starter-connected neurons appeared in the CeM, and most of these labelled CeM cells were immunopositive for CRH, indicating a direct monosynaptic input from CeM CRH cells onto LPGi catecholaminergic neurons.

Fig. 7A-D

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

CEAm (Central amygdalar nucleus, medial part) MBA:559 projects to PGRNl (Paragigantocellular reticular nucleus, lateral part) MBA:978 · Mouse

A retrogradely transported AAV carrying CRH-driven Cre was injected bilaterally into the LPGi together with a Cre-dependent DREADD virus in the CeM; mCherry-tagged receptor expression was then found in CeM neurons, showing that CeM cells send axons to the LPGi.

Figs. 8A-C and 9A-C

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.