Projections from infralimbic medial prefrontal cortex glutamatergic outputs to amygdala mediates opioid induced hyperalgesia in male rats

ILA (Infralimbic area) UBERON:8440033 functionally connects to CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

Blue-light stimulation of ChR2-expressing IL terminals in amygdala slices evoked EPSCs in CeLC neurons that disappeared with TTX, were restored by 4-AP and abolished by CNQX, indicating a monosynaptic glutamatergic input; the same stimulation also produced longer-latency, CNQX- and picrotoxin-sensitive IPSCs, consistent with disynaptic feed-forward inhibition, and in vivo photostimulation of these terminals lowered c-Fos counts in CeLC.

Fig. 3(c)-(e), Fig. 4(b)-(d), Fig. 5(f), (g)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) disagrees. “The functional IL→CeLC excitatory connection is supported, but the attached number (conduction delay 0.0 ms) is not a value the paper reports for this connection.”

ILA (Infralimbic area) UBERON:8440033 functionally connects to BLA (basal amygdaloid nucleus) UBERON:0002887 · Rat

Photostimulation of ChR2-expressing IL axons in slices evoked EPSCs and IPSCs in BLA neurons; unlike the CeLC, these amplitudes and their ratio were unchanged by opioid-induced hyperalgesia.

Sup. Figure 2

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ILA (Infralimbic area) UBERON:8440033 projects to BLA (basal amygdaloid nucleus) UBERON:0002887 · Rat

Viral labelling of CaMKIIa-positive IL neurons revealed heavy terminal fields in the basolateral amygdala, described by the authors as dense projections from IL to BLA.

Results (section on IL-CeLC synaptic transmission), Sup. Figure 1/2

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ILA (Infralimbic area) UBERON:8440033 projects to CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

After injecting CaMKIIa-driven AAV carrying ChR2-EYFP or Cre-EGFP into the right infralimbic cortex, labelled axon terminals were visible in the laterocapsular central amygdala, although the authors note the IL-to-CeLC projection was not abundant.

Fig. 3(b), Fig. 4(a), Fig. 5(b)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ILA (Infralimbic area) UBERON:8440033 synapses onto CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

An anterograde transsynaptic AAV1/2 expressing Cre-EGFP under the CaMKIIa promoter was placed in the right infralimbic cortex; four weeks later EGFP appeared in a subset of CeLC neurons, indicating they receive direct synaptic input from IL glutamatergic pyramidal cells.

Fig. 3(a), (b)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.