Posterodorsal Medial Amygdala Urocortin-3, GABA, and Glutamate Mediate Suppression of LH Pulsatility in Female Mice

MEA (Medial amygdalar nucleus) MBA:403 projects to PVH (Paraventricular hypothalamic nucleus) MBA:38 · Mouse

Cre-dependent AAV carrying ChR2-EYFP injected into the posterodorsal medial amygdala of Ucn3-cre-tdTomato mice produced YFP-labelled axonal fibres within the hypothalamic paraventricular nucleus, showing that Ucn3 cells of the MePD send axons to the PVN; the authors note some fibres might reflect non-specific ChR2 expression.

Fig. 5, A-Aiii; Results, final section

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MEA (Medial amygdalar nucleus) MBA:403 functionally connects to PVH (Paraventricular hypothalamic nucleus) MBA:38 · Mouse

Blue-light stimulation (20 Hz, 15 mW) of ChR2-expressing MePD Ucn3 axon terminals within the PVN raised circulating corticosterone relative to control virus animals, indicating that the MePD Ucn3 input drives PVN-dependent HPA axis output; no such change occurred in AAV-YFP controls.

Fig. 2; Results, 'Effect of MePD Urocortin-3 Terminal Stimulation in the PVN on Dynamic CORT Secretion'

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