Specific Targeting of the Basolateral Amygdala to Projectionally Defined Pyramidal Neurons in Prelimbic and Infralimbic Cortex

ILA5 (Infralimbic area, layer 5) MBA:827 projects to PAG (Periaqueductal gray) MBA:795 · Mouse

Retrograde tracer (Retrobeads) injected into the PAG labelled cortico-PAG pyramidal neurons in layer 5 of IL cortex.

Fig. 2B-C; Fig. 4B-C

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PL (Prelimbic area) MBA:972 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

CTB-AlexaFluor 647 injected into the BLA retrogradely labelled corticoamygdalar neurons in layer 2 of PL cortex.

Fig. 3I-J; Fig. 4A,C

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to PL (Prelimbic area) MBA:972 · Mouse

AAV-ChR2-YFP injected into the BLA labelled axons in PL cortex. Axon fluorescence was higher in layer 2 of PL than of IL, and was limited in PL layer 5.

Fig. 1D-G; Results, first section

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BLA (Basolateral amygdalar nucleus) MBA:295 projects to ILA (Infralimbic area) MBA:44 · Mouse

After AAV-ChR2-YFP injection into the BLA, YFP-labelled axons appeared in IL cortex. They were uniform across layer 5 and sparse in layer 2.

Fig. 1D-G; Results, first section

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to PL (Prelimbic area) MBA:972 · Mouse

Optogenetic stimulation of BLA axons evoked EPSCs and IPSCs in L5 PL cortico-PAG neurons; blockers reduced these to a small monosynaptic EPSC and removed the IPSC. L2 PL corticoamygdalar neurons received strong monosynaptic EPSCs and could be driven to fire action potentials.

Fig. 2H-K; Fig. 3A-D, J-L

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PL5 (Prelimbic area, layer 5) MBA:363 projects to PAG (Periaqueductal gray) MBA:795 · Mouse

Retrograde tracer injected into the PAG labelled cortico-PAG neurons in layer 5 of PL cortex.

Fig. 2B-C

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to ILA (Infralimbic area) MBA:44 · Mouse

Light activation of ChR2+ BLA axons in slices evoked EPSCs in IL neurons that mostly persisted in TTX/4-AP, indicating monosynaptic excitation. IPSCs were abolished by the blockers, so the inhibition was polysynaptic. Monosynaptic input was larger in L5 cortico-PAG neurons than in L3/5 corticoamygdalar neurons, and larger in L3/5 than in L2 corticoamygdalar neurons.

Fig. 2D-G; Fig. 3B-D, J-L; Fig. 4D-I

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ILA (Infralimbic area) MBA:44 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

CTB injected into the BLA retrogradely labelled corticoamygdalar neurons in IL cortex, in layer 2 and in layers 3/5.

Fig. 3I-J; Fig. 4A,C,G

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.