Chronic Ethanol Differentially Modulates Glutamate Release from Dorsal and Ventral Prefrontal Cortical Inputs onto Rat Basolateral Amygdala Principal Neurons

ILA (Infralimbic area) UBERON:8440033 projects to LA (lateral amygdaloid nucleus) UBERON:0002886 · Rat

Ventral mPFC injections left the lateral amygdaloid nucleus with little to no terminal labelling.

Results, first section (Fig. 1D1)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ILA (Infralimbic area) UBERON:8440033 projects to BLA (basal amygdaloid nucleus) UBERON:0002887 · Rat

AAV-ChR2-EYFP injected into the ventral mPFC produced robust fluorescent terminal labelling in the basolateral amygdala, with a pattern similar to that of the dorsal mPFC injections and concentrated in the anterior BLA.

Fig. 1D1; Results, first section

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PL (Prelimbic area) UBERON:8440032 projects to LA (lateral amygdaloid nucleus) UBERON:0002886 · Rat

Virtually no YFP-labelled terminals from the dorsal mPFC injections were found in the lateral amygdaloid nucleus.

Results, first section (Fig. 1C1)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PL (Prelimbic area) UBERON:8440032 functionally connects to Rat basal amygdaloid nucleus principal neurons nt-gv1k4bnh04 · Rat

Blue-light activation of ChR2-expressing dorsal mPFC fibres near the stria terminalis evoked EPSCs in BLA principal neurons; these were abolished by TTX and restored by 4-AP (indicating monosynaptic transmission) and blocked by the AMPA antagonist DNQX, establishing a glutamatergic connection.

Fig. 1C2; Fig. 6A3

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PL (Prelimbic area) UBERON:8440032 projects to CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

Labelled axons from the dorsal mPFC passed just dorsal to the central amygdala but essentially no terminals were observed within the CeA itself.

Results, first section (Fig. 1C1)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ILA (Infralimbic area) UBERON:8440033 projects to CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

No appreciable ventral mPFC terminal labelling was detected inside the central amygdala.

Results, first section (Fig. 1D1)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ILA (Infralimbic area) UBERON:8440033 functionally connects to Rat basal amygdaloid nucleus principal neurons nt-gv1k4bnh04 · Rat

Optical stimulation of ventral mPFC terminals evoked GABAergic IPSCs in BLA principal neurons with longer latencies than the EPSCs; these IPSCs were attenuated by picrotoxin and by DNQX, consistent with recruitment of local GABAergic neurons rather than direct inhibition.

Fig. 6B3, B4

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) disagrees. “The IPSCs are DNQX-sensitive local circuit responses, so the vmPFC input itself is not inhibitory as the sign asserts.”

ILA (Infralimbic area) UBERON:8440033 functionally connects to Rat basal amygdaloid nucleus principal neurons nt-gv1k4bnh04 · Rat

Optical stimulation of ventral mPFC ChR2-expressing terminals produced EPSCs in BLA principal neurons that persisted in TTX plus 4-AP (about 81% of baseline amplitude, i.e. largely monosynaptic) and were eliminated by DNQX, showing glutamatergic input.

Fig. 1D2; Fig. 6B3

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PL (Prelimbic area) UBERON:8440032 functionally connects to Rat basal amygdaloid nucleus principal neurons nt-gv1k4bnh04 · Rat

Light activation of dorsal mPFC terminals also evoked GABAergic IPSCs in BLA principal neurons recorded at 0 mV; their onset was much later than the EPSCs and they were blocked by picrotoxin as well as by DNQX, indicating a polysynaptic local inhibitory circuit recruited by this input.

Fig. 6A3, A4

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) disagrees. “The dmPFC-evoked IPSCs are blocked by DNQX, indicating a polysynaptic feedback circuit rather than an inhibitory dmPFC connection.”

PL (Prelimbic area) UBERON:8440032 projects to BLA (basal amygdaloid nucleus) UBERON:0002887 · Rat

After AAV-ChR2-EYFP injection into the dorsal mPFC, strong YFP-labelled axons were seen running along the stria terminalis and within the basolateral amygdala, mostly in its anterior subdivision.

Fig. 1C1; Results, first section

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.