Selective activation of the hypothalamic orexinergic but not melanin-concentrating hormone neurons following pilocarpine-induced seizures in rats

MEA (medial amygdaloid nucleus) UBERON:0002892 projects to LHA (Lateral hypothalamic area) UBERON:0002430 · Rat

CTb injected into the lateral hypothalamus retrogradely labelled neurons in the ipsilateral amygdala, including the medial amygdala. A larger share of Fos+ medial amygdala neurons was CTb-labelled in seizure rats (about 33%) than in controls (about 12%).

Fig. 6A,D; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MEA (medial amygdaloid nucleus) UBERON:0002892 projects to LZ (Hypothalamic lateral zone) UBERON:0002273 · Rat

After CTb injection into the perifornical region, retrogradely labelled neurons appeared in the ipsilateral amygdala, with labelled cells (especially seizure-activated ones) concentrated in medial subnuclei including MeA. About 56% of Fos+ neurons were CTb co-labelled in seizure rats versus about 11% in controls.

Fig. 5A,D; Results, 'Amygdala neurons might transmit epileptic activity...'

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “The 56% vs 11% co-labelling figures are for amygdala neurons overall after perifornical injection, not for MeA specifically.”

BMA (medial part of basal amygdaloid nucleus) UBERON:0002889 projects to LZ (Hypothalamic lateral zone) UBERON:0002273 · Rat

CTb injected into the perifornical region retrogradely labelled amygdala neurons. CTb/Fos double-labelled cells in seizure rats were concentrated in medial subnuclei, including BMA.

Fig. 5D; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CEA (central amygdaloid nucleus) UBERON:0002883 projects to LZ (Hypothalamic lateral zone) UBERON:0002273 · Rat

After perifornical CTb injection, retrogradely labelled amygdala neurons that were also Fos+ after seizures were concentrated in medial subnuclei, including CeA.

Fig. 5D; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MEA (medial amygdaloid nucleus) UBERON:0002892 projects to LHA (Lateral hypothalamic area) UBERON:0002430 · Rat

BDA injected into the medial amygdala labelled fibers and terminal boutons in the hypothalamus. These closely contacted, and sometimes encircled, seizure-activated orexin neurons.

Fig. 7A,B

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

LHA (Lateral hypothalamic area) UBERON:0002430 projects to MEA (medial amygdaloid nucleus) UBERON:0002892 · Rat

CTb injected into the medial amygdala retrogradely labelled many orexin-immunoreactive neurons in the lateral and perifornical hypothalamus, some of them Fos+ after seizures. This indicates a reciprocal projection back to the medial amygdala.

Fig. 7C,D

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.