Kappa Opioid Receptor Blockade in the Amygdala Mitigates Pain Like-Behaviors by Inhibiting Corticotropin Releasing Factor Neurons in a Rat Model of Functional Pain

PB (Parabrachial nucleus) UBERON:0007634 functionally connects to Rat central amygdala corticotropin-releasing hormone-expressing neurons nt-sexhekqxaa · Rat

Blue-light activation of ChR2-expressing PB terminals in slices from Crh-Cre rats evoked EPSCs in every mCherry-labelled CeA CRF neuron tested. AP5/CNQX blocked these EPSCs. The same stimulation also evoked glutamate-dependent feedforward IPSCs that bicuculline blocked; yellow light evoked no response.

Fig. 6D,E; Results 2.4

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PB (Parabrachial nucleus) UBERON:0007634 projects to CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

After CaMKIIa-ChR2-eYFP virus injection into the external lateral parabrachial nucleus, labelled terminals were found in the central amygdala, in the lateral division and more prominently in the capsular division.

Fig. 2A,B; Methods 5.7.3, 5.8

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PB (Parabrachial nucleus) UBERON:0007634 functionally connects to Rat central amygdala corticotropin-releasing hormone-expressing neurons nt-sexhekqxaa · Rat

Focal electrical stimulation of the PB fiber tract dorsomedial to the CeA evoked glutamate receptor-dependent EPSCs, plus feedforward IPSCs, in all CeA CRF neurons tested in brain slices.

Fig. 6F,G; Results 2.4

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.