Adolescent alcohol exposure promotes mechanical allodynia and alters synaptic function at inputs from the basolateral amygdala to the prelimbic cortex

PL (Prelimbic area) UBERON:8440032 projects to PAG (Periaqueductal gray) UBERON:0003040 · Rat

After green retrobeads were injected into the left vlPAG, labelled pyramidal neurons were found in the ipsilateral PrL cortex and used as PAG-projecting cells for recordings.

Fig. 3C-D; Results, PrLPAG intrinsic excitability section

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BLA (basal amygdaloid nucleus) UBERON:0002887 projects to PL (Prelimbic area) UBERON:8440032 · Rat

An AAV expressing ChR2-EYFP was injected into the BLA, and EYFP-labelled BLA axon terminals were imaged in the PrL cortex.

Fig. 3C-D

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BLA (basal amygdaloid nucleus) UBERON:0002887 functionally connects to PL (Prelimbic area) UBERON:8440032 · Rat

Light stimulation of ChR2-expressing BLA terminals in PrL slices evoked excitatory currents in retrobead-labelled PAG-projecting neurons. These currents persisted in TTX/4-AP, consistent with monosynaptic input, and had an APV-sensitive NMDA component. The same stimulation also evoked feedforward inhibitory currents, which were reduced after adolescent ethanol exposure.

Figs. 5-7

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BLA (basal amygdaloid nucleus) UBERON:0002887 functionally connects to PL (Prelimbic area) UBERON:8440032 · Rat

Optogenetic activation of BLA terminals evoked excitatory currents in mCherry-tagged PrL PV interneurons. The currents included monosynaptic AMPA and APV-sensitive NMDA components and asynchronous EPSCs recorded under TTX/4-AP, and they were reduced by adolescent ethanol exposure.

Figs. 9-11

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.