Alcohol attenuates CRF-induced excitatory effects from the extended amygdala to dorsostriatal cholinergic interneurons

BST (Bed nuclei of the stria terminalis) UBERON:0001880 projects to CP (Caudoputamen) UBERON:0005383 · Rat

Retrograde AAVretro-DIO-GFP injected in the dorsal striatum of CRF-Cre;tdTomato rats labelled GFP+/tdTomato+ cells in the BNST, demonstrating a CRF-positive BNST projection to the dorsal striatum; both the proportion (p<0.001) and density (p<0.01) of dorsal striatum-projecting CRF neurons were greater in BNST than in CeA (8 sections from 5 rats).

Fig. 3A, 3B, 3D–3F

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BST (Bed nuclei of the stria terminalis) MBA:351 synapses onto CP (Caudoputamen) MBA:672 · Mouse

Monosynaptic rabies tracing from dorsal striatal cholinergic interneurons in ChAT-Cre;D1-tdTomato mice produced GFP-labelled presynaptic neurons in the bed nucleus of the stria terminalis, and the density of labelled cells there exceeded that in the central amygdala (15 sections from 4 mice).

Fig. 1A, 1B, 1D, 1E

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BST (Bed nuclei of the stria terminalis) MBA:351 functionally connects to CP (Caudoputamen) MBA:672 · Mouse

Chrimson expressed in CRF-Cre neurons of the BNST (injected together with CeA) allowed optical activation of their striatal fibres, which produced fast optically evoked IPSCs in cholinergic interneurons; TTX abolished and TTX+4-AP restored the response, and bicuculline blocked it, indicating a monosynaptic GABAergic connection.

Fig. 3G–3J

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SS (Somatosensory areas) MBA:453 synapses onto CP (Caudoputamen) MBA:672 · Mouse

Somatosensory cortex contained neurons labelled by monosynaptic rabies tracing initiated in dorsal striatal cholinergic interneurons.

Results, first section (Fig. 1)

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PF (Parafascicular nucleus) MBA:930 synapses onto CP (Caudoputamen) MBA:672 · Mouse

The parafascicular thalamic nucleus contained rabies-labelled neurons presynaptic to dorsal striatal cholinergic interneurons.

Results, first section (Fig. 1)

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STR (Striatum) MBA:477 synapses onto CP (Caudoputamen) MBA:672 · Mouse

Rabies-based monosynaptic tracing from striatal cholinergic interneurons labelled presynaptic neurons within the striatum itself.

Results, first section (Fig. 1)

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MB (Midbrain) MBA:313 synapses onto CP (Caudoputamen) MBA:672 · Mouse

Midbrain neurons were labelled as presynaptic partners of dorsal striatal cholinergic interneurons in the rabies tracing experiment.

Results, first section (Fig. 1)

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CEA corticotropin-releasing hormone-expressing neurons nt-vpnb3tg80r functionally connects to CP (Caudoputamen) MBA:672 · Mouse

AAV-FLEX-Chrimson-tdTomato was injected into both CeA and BNST of CRF-Cre;ChAT-eGFP mice; light stimulation of the labelled fibres in dorsal striatum evoked short-latency synaptic currents in GFP+ cholinergic interneurons that were abolished by TTX, restored by TTX+4-AP (indicating monosynaptic transmission) and blocked by the GABA-A antagonist bicuculline.

Fig. 3G–3J

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PALd (Pallidum, dorsal region) MBA:818 synapses onto CP (Caudoputamen) MBA:672 · Mouse

Globus pallidus neurons were retrogradely labelled by monosynaptic rabies tracing from dorsal striatal cholinergic interneurons.

Results, first section (Fig. 1)

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CL (Central lateral nucleus of the thalamus) MBA:575 synapses onto CP (Caudoputamen) MBA:672 · Mouse

The central lateral (centrolateral) thalamic nucleus was among regions with rabies-labelled neurons presynaptic to dorsal striatal cholinergic interneurons.

Results, first section (Fig. 1)

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ACA (Anterior cingulate area) MBA:31 synapses onto CP (Caudoputamen) MBA:672 · Mouse

Among the regions containing rabies-labelled neurons presynaptic to dorsal striatal cholinergic interneurons, the authors list the cingulate cortex.

Results, first section (Fig. 1)

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Rat central amygdala corticotropin-releasing hormone-expressing neurons nt-sexhekqxaa projects to CP (Caudoputamen) UBERON:0005383 · Rat

After infusing a retrograde Cre-dependent AAV expressing GFP into the dorsal striatum of CRF-Cre;tdTomato rats, GFP-labelled cells overlapping with tdTomato (CRF+) were found in the central amygdala, showing that CeA CRF neurons send axons to the dorsal striatum; the proportion and density of such projecting CRF neurons was lower in CeA than in BNST (9 sections from 5 rats).

Fig. 3A, 3C–3F

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CEA (Central amygdalar nucleus) MBA:536 synapses onto CP (Caudoputamen) MBA:672 · Mouse

Cre-dependent helper viruses plus EnvA-pseudotyped rabies-GFP targeted to cholinergic interneurons of the dorsomedial striatum in ChAT-Cre;D1-tdTomato mice labelled presynaptic neurons in the central amygdala, indicating a direct monosynaptic input onto striatal CINs. Labelling density in CeA was lower than in BNST (Mann-Whitney U = 4.000, p<0.001; 13 sections from 4 mice).

Fig. 1A–C, 1E

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MO (Somatomotor areas) MBA:500 synapses onto CP (Caudoputamen) MBA:672 · Mouse

Rabies-labelled presynaptic neurons of dorsal striatal cholinergic interneurons were found in motor cortex.

Results, first section (Fig. 1)

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