Switching of delta opioid receptor subtypes in central amygdala microcircuits is associated with anxiety states in pain

BLA (Basolateral amygdalar nucleus) MBA:295 projects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

After injecting Cre-dependent ChR2-mCherry or eNpHR3.0-EYFP virus into the BLA of CaMKII-Cre mice, labelled axonal fibers were present in the CeA, where optical fibers were implanted and terminal photostimulation was performed.

Fig. 3, A, B and H; Experimental procedures

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “The cited panels and text show viral expression only at the BLA injection site; the paper never shows or describes labelled BLA fibers in the CeA as tracing evidence.”

BLA glutamatergic principal neurons nt-wprma5fcrr functionally connects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

Cre-dependent ChR2 was expressed in BLA excitatory neurons of CaMKII-Cre mice; blue-light stimulation of their terminals in the CeA elicited EPSCs in CeA neurons that were abolished by the AMPA receptor blocker DNQX, indicating a glutamatergic BLA-to-CeA input. These light-evoked EPSCs were suppressed by the DOR1 agonist DPDPE in normal and 4-h CFA mice but by neither DPDPE nor deltorphin II in 7-day CFA mice.

Fig. 3, A–F

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PB (Parabrachial nucleus) MBA:867 functionally connects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

ChR2 expressed in PBN excitatory neurons of CaMKII-Cre mice allowed light stimulation of PBN terminals in the CeA, which reliably evoked EPSCs in CeA neurons that were blocked by DNQX. The DOR1 agonist DPDPE reduced these EPSCs in normal and 4-h CFA mice, whereas in 7-day CFA mice the DOR2 agonist deltorphin II, but not DPDPE, reduced them.

Fig. 4, A–E

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PB (Parabrachial nucleus) MBA:867 projects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

Cre-dependent ChR2-mCherry injected into the PBN of CaMKII-Cre mice labelled cell bodies in the PBN and produced abundant mCherry-positive axonal fibers in the CeA.

Fig. 4, A and B

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.