Ventral hippocampal afferents to the nucleus accumbens regulate susceptibility to depression

ILA (Infralimbic area) MBA:44 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

Retrograde AAV2/5-CaMKIIa-EYFP injected into nucleus accumbens labelled neurons in medial prefrontal cortex, identifying an mPFC population projecting to NAc.

Fig. 1i,j

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BLA (Basolateral amygdalar nucleus) MBA:295 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

Retrograde AAV2/5-CaMKIIa-EYFP injected into nucleus accumbens labelled neurons in the amygdala, identifying NAc-projecting amygdala neurons.

Fig. 1k,l

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BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to ACB (Nucleus accumbens) MBA:56 · Mouse

Light activation of ChR2-expressing basolateral amygdala terminals in NAc shell slices elicited EPSCs in medium spiny neurons, including paired-pulse responses and responses to 1 Hz and 4 Hz stimulation, showing a glutamatergic amygdala input to NAc.

Fig. 1q,r; Fig. 2d; Fig. 3d

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SUB (Subiculum) MBA:502 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

A retrogradely transported AAV2/5-CaMKIIa-EYFP vector injected into the nucleus accumbens labelled EYFP-positive neurons in ventral hippocampus, which were then assessed for EGR1 immunoreactivity, demonstrating vHIP neurons that project to NAc.

Fig. 1g,h; Methods (Stereotaxic surgery and viral vectors)

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ILA (Infralimbic area) MBA:44 functionally connects to ACB (Nucleus accumbens) MBA:56 · Mouse

Optical stimulation of ChR2-expressing mPFC axon terminals in NAc shell slices evoked EPSCs in medium spiny neurons, with paired-pulse responses and reliable responses to 1 Hz and 4 Hz light trains, consistent with a glutamatergic mPFC input to NAc.

Fig. 1o,p; Fig. 2c; Fig. 3c

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SUB (Subiculum) MBA:502 functionally connects to ACB (Nucleus accumbens) MBA:56 · Mouse

Blue-light stimulation of ChR2-expressing vHIP terminals in NAc shell slices reliably evoked EPSCs in accumbens medium spiny neurons (including paired-pulse and 1 Hz/4 Hz trains), and the optically evoked currents were abolished by glutamate receptor antagonists, indicating a glutamatergic vHIP-to-NAc connection.

Fig. 1m,n; Fig. 2b; Fig. 3b; Supplementary Fig. 2c

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.