Medial prefrontal D1 dopamine neurons control food intake

PL (Prelimbic area) MBA:972 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

ChR2-eYFP expressed in prefrontal D1 neurons produced prominent labelled axons in the medial nucleus accumbens, both shell and core.

Fig. 5a legend; Supplemental Fig. 7a,b; Results, 'Downstream targets of mPFC stimulation'

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PL (Prelimbic area) MBA:972 functionally connects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Photostimulation (20 Hz) of ChR2-expressing D1 neurons in the medial prefrontal cortex raised Fos immunoreactivity in the ipsilateral medial basolateral amygdala relative to the contralateral side and to Cre-negative controls. Directly illuminating the prefrontal D1 axon terminals in the medial basolateral amygdala likewise increased Fos there on the stimulated side.

Fig. 5b,c; Fig. 6b,c

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PL (Prelimbic area) MBA:972 functionally connects to BLA parvalbumin-expressing interneurons nt-fwck8mp6aj · Mouse

Co-staining showed essentially no overlap between the Fos signal evoked in the medial basolateral amygdala by prefrontal D1 photostimulation and parvalbumin: only about 5% of Fos-positive nuclei were PV-positive, so prefrontal D1 activation did not appreciably recruit these interneurons.

Fig. 5e,f

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PL (Prelimbic area) MBA:972 functionally connects to ACB (Nucleus accumbens) MBA:56 · Mouse

Despite dense prefrontal D1 axon labelling there, 20 Hz photostimulation of prefrontal D1 neurons produced no detectable change in Fos immunoreactivity in the nucleus accumbens.

Supplemental Fig. 7d,e; Results, 'Downstream targets of mPFC stimulation'

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PL (Prelimbic area) MBA:972 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Cre-dependent ChR2-eYFP expressed in D1-receptor neurons of the medial prefrontal cortex (prelimbic and infralimbic) of Drd1a-cre mice labelled axons that were prominent in the caudomedial basolateral amygdala. A Cre-dependent synaptobrevin-eGFP virus injected in the same prefrontal site produced punctate presynaptic labelling in the same amygdala territory, with puncta lying within 5 microns of CaMKII-positive cell bodies.

Fig. 5a,g,h; Results, 'Downstream targets of mPFC stimulation'

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PL (Prelimbic area) MBA:972 projects to CP (Caudoputamen) MBA:672 · Mouse

Axons of ChR2-eYFP labelled prefrontal D1 neurons were prominent in the dorsomedial striatum.

Supplemental Fig. 7a,b; Results, 'Downstream targets of mPFC stimulation'

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PL (Prelimbic area) MBA:972 projects to LHA (Lateral hypothalamic area) MBA:194 · Mouse

Only sparse ChR2-eYFP labelled axons from prefrontal D1 neurons were seen in the lateral hypothalamus.

Supplemental Fig. 7c; Results, 'Downstream targets of mPFC stimulation'

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PL (Prelimbic area) MBA:972 functionally connects to BLA glutamatergic principal neurons nt-wprma5fcrr · Mouse

About 85% of the Fos-positive nuclei driven in the medial basolateral amygdala by prefrontal D1 photostimulation were CaMKII-positive (roughly 20% of CaMKII cells in this territory). Silencing these CaMKII neurons with halorhodopsin and yellow light abolished the feeding increase produced by blue-light stimulation of the prefrontal D1 terminals, indicating that the prefrontal input acts through these amygdala glutamatergic cells.

Fig. 5d,f; Fig. 6g-i

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.