Conditional deletion of Cc2d1a increases irritability-like behavior by decreasing oxytocin expression via the prelimbic-hypothalamic pathway

PL (Prelimbic area) MBA:972 projects to PVH (Paraventricular hypothalamic nucleus) MBA:38 · Mouse

After bilateral injection of a Cre-dependent retrograde AAV (AAVrg-hSyn-DIO-mCherry) into the PVN of Emx1-Cre mice, retrogradely labelled cortical neurons were found in the prelimbic cortex, indicating prelimbic projections to the PVN.

Fig. 6A, B

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ACA (Anterior cingulate area) MBA:31 projects to PVH (Paraventricular hypothalamic nucleus) MBA:38 · Mouse

Cre-dependent retrograde AAV injected into the PVN of Emx1-Cre mice also labelled neurons in the anterior cingulate cortex, showing that this cortical area projects to the PVN.

Fig. 6A, B

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PL (Prelimbic area) MBA:972 synapses onto PVH (Paraventricular hypothalamic nucleus) MBA:38 · Mouse

In the same anterograde transsynaptic labelling experiment, the transsynaptically labelled PVN neurons were not immunoreactive for oxytocin, so no direct prelimbic input onto PVN oxytocin neurons was detected.

Fig. 6D, F

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PL (Prelimbic area) MBA:972 synapses onto PVH (Paraventricular hypothalamic nucleus) MBA:38 · Mouse

Anterograde transsynaptic labelling with AAV1-hSyn-Cre in the PrL of Ai14 reporter mice (with AAV5-hSyn-DIO-EGFP in the PVN) produced labelled PVN neurons that co-expressed the GABAergic marker GAD67, indicating that prelimbic axons contact PVN inhibitory neurons.

Fig. 6C–F

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.