PTEN in somatostatin neurons regulates fear and anxiety and is required for inhibitory synaptic connectivity within central amygdala

CEAl somatostatin-expressing neurons nt-t6ymz01hv4 functionally connects to CEAl (Central amygdalar nucleus, lateral part) MBA:551 · Mouse

Single-cell two-photon activation of CeL SOM+ neurons evoked IPSCs in patched SOM− neurons: 160 of 681 activated cells were connected in wild-type mice and 63 of 557 in SOM-PTEN-KO mice. IPSCs were also smaller in the knockout.

Fig. 3k; Fig. 4c; Results 2.4

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CEAl (Central amygdalar nucleus, lateral part) MBA:551 functionally connects to CEAl somatostatin-expressing neurons nt-t6ymz01hv4 · Mouse

Photoactivating single tdTomato-negative (SOM−) CeL neurons produced IPSCs in patched SOM+ neurons. In wild-type mice 107 of 204 activated cells were connected, and connectivity fell to 73 of 333 in SOM-PTEN-KO mice.

Fig. 3i; Fig. 4b; Results 2.4

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to CEAl somatostatin-expressing neurons nt-t6ymz01hv4 · Mouse

Blue-light activation of ChR2-expressing BLA axons in slices evoked excitatory postsynaptic currents with AMPA and NMDA components in patched CeL somatostatin neurons. Peak currents trended larger and the AMPA/NMDA ratio was higher in SOM-PTEN-KO mice, while paired-pulse ratio did not change.

Fig. 2a-d; Results 2.2

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.