Mouse
PB (Parabrachial nucleus) functionally connects to CEA (Central amygdalar nucleus)
3 claims from 3 sources: 3 found, 0 tested and absent, 0 ambiguous. Evidence: electrical stimulation, optogenetic circuit mapping.
The claims behind it
Stimulating the fiber bundle of LPB origin running dorsomedial to the CeA evoked short-latency excitatory postsynaptic currents in CeC/L neurons recorded at -70 mV; these responses were larger in formalin-treated than saline-treated mice and, unlike BLA-evoked responses, were unaffected by pregabalin. Both LPB- and BLA-evoked EPSCs could be recorded in the same CeC/L neurons, showing convergence of the two inputs.
DOI 10.1016/j.ynpai.2021.100078 · PubMed 34877437 · PMC8628014, Figs. 1B, 4, 5; ResultsMade by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.
In acute slices, red-light activation of C1ChrimsonSA expressed in parabrachial axons evoked excitatory postsynaptic currents in whole-cell recorded CeA neurons (isolated with picrotoxin), and photoactivation of presynaptically targeted bPAC potentiated these currents with a concurrent drop in paired-pulse ratio, indicating a functional monosynaptic excitatory PB-to-CeA input with presynaptically regulated release.
DOI 10.1016/j.crmeth.2024.100740 · PubMed 38521059 · PMC11045876, Figs. 4A–4D, 5A–5BMade by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.
ChR2 expressed in PBN excitatory neurons of CaMKII-Cre mice allowed light stimulation of PBN terminals in the CeA, which reliably evoked EPSCs in CeA neurons that were blocked by DNQX. The DOR1 agonist DPDPE reduced these EPSCs in normal and 4-h CFA mice, whereas in 7-day CFA mice the DOR2 agonist deltorphin II, but not DPDPE, reduced them.
DOI 10.1016/j.jbc.2021.100277 · PubMed 33428940 · PMC7948800, Fig. 4, A–EMade by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.