The pPVTglu- CeAGABA circuit is involved in regulating hyperalgesia in the chronic NTG mouse model in males

PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to FS (Fundus of striatum) MBA:998 · Mouse

Anterograde tracing from pPVT glutamatergic neurons labelled axon terminals in the interstitial nucleus of the posterior limb of the anterior commissure.

Fig. 3A-B

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PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to CEAc (Central amygdalar nucleus, capsular part) MBA:544 · Mouse

Anterograde tracing of activity-tagged pPVT neurons in FosTRAP2 mice revealed mRuby-labelled fibre terminals in the capsular part of the CeA, with greater terminal fluorescence in NTG-treated mice than vehicle controls.

Fig. 4F-H

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PVT (Paraventricular nucleus of the thalamus) MBA:149 synapses onto BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Anterograde transsynaptic Cre from the pPVT produced tdTomato-positive postsynaptic somata in the basolateral amygdala.

Fig. 3C-D

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PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Anterograde, non-transsynaptic tracing from pPVT Vglut2+ neurons produced mRuby-labelled fibre terminals in the bed nucleus of the stria terminalis.

Fig. 3A-B

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PVT (Paraventricular nucleus of the thalamus) MBA:149 synapses onto CEA (Central amygdalar nucleus) MBA:536 · Mouse

Anterograde transsynaptic Cre injected in the pPVT labelled postsynaptic cell bodies in the central amygdala of Ai9 mice; a second experiment with transsynaptic Flp from the pPVT combined with a Cre/Flp-dependent vector in VGAT-Cre mice selectively labelled CeA GABAergic neurons receiving pPVT input.

Fig. 3C-D; Fig. 7G-I

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PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

A retrograde Cre-dependent EGFP virus injected unilaterally into the CeA of Vglut2-Cre mice labelled Vglut2+ neurons in the ipsilateral pPVT across several anterior-posterior levels.

Fig. 4D-E

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PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

A Cre-dependent anterograde tracer expressing mGFP and synaptophysin-mRuby was injected into the pPVT of Vglut2-Cre mice; mRuby-labelled axon terminals from pPVT glutamatergic neurons were seen in the nucleus accumbens three weeks later.

Fig. 3A-B

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PVT (Paraventricular nucleus of the thalamus) MBA:149 synapses onto FS (Fundus of striatum) MBA:998 · Mouse

Transsynaptic Cre spread from the pPVT labelled postsynaptic neuronal cell bodies in the IPAC of Ai9 mice.

Fig. 3C-D

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PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

Cre-dependent anterograde tracing in Vglut2-Cre mice showed mRuby-labelled terminals from pPVT glutamatergic neurons in the central amygdala.

Fig. 3A-B

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PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to CEAm (Central amygdalar nucleus, medial part) MBA:559 · Mouse

Terminals from pPVT neurons labelled with synaptophysin-mRuby were also quantified in the medial part of the CeA, where terminal fluorescence did not differ between NTG and vehicle groups.

Fig. 4G-H

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PVT (Paraventricular nucleus of the thalamus) MBA:149 functionally connects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

Chrimson was expressed in pPVT CaMKIIα+ neurons and GCaMP6s in CeA GABAergic neurons of VGAT-Cre mice; red-light stimulation of pPVT terminals within the CeA increased the calcium signal of CeA GABAergic neurons, with a larger area under the curve than in mCherry controls, indicating a predominantly excitatory functional connection.

Fig. 6A-E

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PVT (Paraventricular nucleus of the thalamus) MBA:149 synapses onto BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Anterograde transsynaptic Cre delivery from the pPVT in Ai9 mice labelled postsynaptic somata in the bed nucleus of the stria terminalis.

Fig. 3C-D

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Synaptophysin-mRuby labelled terminals of pPVT Vglut2+ neurons were observed in the basolateral amygdala.

Fig. 3A-B

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to CEAl (Central amygdalar nucleus, lateral part) MBA:551 · Mouse

mRuby-labelled terminals from activity-tagged pPVT neurons were present in the lateral part of the CeA, and were denser in the chronic migraine model group.

Fig. 4F-H

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PVT (Paraventricular nucleus of the thalamus) MBA:149 synapses onto ACB (Nucleus accumbens) MBA:56 · Mouse

High-titre anterograde transsynaptic AAV2/1-hSyn-Cre injected into the pPVT of Ai9 reporter mice produced tdTomato-labelled postsynaptic cell bodies in the nucleus accumbens.

Fig. 3C-D

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.